Supplementary photomicrographs of VMpo

Identification of VMpo

These color figures document the cytoarchitectonic (thionin staining) and immunohistochemical (calbindin fiber labeling) identification of VMpo.  They supplement the documentation published in the recent article entitled "Distribution of trigeminothalamic and spinothalamic lamina I terminations in the macaque", Journal of Comparative Neurology 477(2): 119-148, 2004.

For each figure, a low-resolution JPEG file (150dpi) is available for quick viewing, and a high resolution TIFF file (300dpi) or PDF is available for download. Abbreviations are used as in the published article. 

Figures 1-6 document the cytoarchitectonic and immunohistochemical identification of VMpo in the macaque monkey. They supplement the documentation published in the article entitled "Distribution of trigeminothalamic and spinothalamic lamina I terminations in the macaque", Journal of Comparative Neurology 477(2): 119-148, 2004.

Figures 1 and 2 show the calbindin-positive fiber labeling in VMpo in serial coronal sections from case M125 at low (Fig. 1) and high (Fig. 2) magnification. The two pages (a, b) show the complete posterior-to-anterior series of 12 consecutive 1-in-3 35-µm-thick coronal sections across the entire extent of VMpo (1295 µm). Annotation for these figures is provided in Figure 13 of the original research article. Scale bar = 1 mm. 
 
Figure 3 shows four selected calbindin-stained sections from Figure 2 paired with the adjacent thionin-stained sections for direct cytoarchitectonic comparison. The individual sections are identified with the same labels used for each panel in Figures 1 and 2.

Figures 4 and 5 show the cytoarchitecture of VMpo in the standard brain M18. The complete posterior-to-anterior series of 12 consecutive 1-in-4 thionin-stained 40-µm-thick coronal sections show the entire extent of VMpo (2240 µm) at low (Fig. 4) and high (Fig. 5) magnification. (One section was omitted between panels A and B). Scale bar = 1 mm. A CD is available upon request with a psd file that shows an overlay layer with a cytoarchitectonic chart of the topographic lamina I terminal labeling illustrated in Figure 11 of the JCN article over the photomicrographs of Figure 4. 

Figure 6 shows a complete series of alternate serial (1-in-2 50-µm-thick) sections stained for Nissl (thionin) and calbindin from case M155. The drawings in the left column (A) delimit the cytoarchitecture of the adjacent thionin section for each calbindin-stained section in the right column (C). The best thionin-stained section for each pair is shown in the middle column (B). The numbers at the top of each image are section numbers (top to bottom = posterior to anterior).