Barrow Neurological Institute of St. Joseph's Hospital and Medical Center, Phoenix, Arizona
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Ronald J. Lukas, PhD
Lukas RJ, Hanley MR, Bennett EL (1978) Properties of radiolabeled(alpha)bungarotoxin derivatives and their interaction with nicotinic acetylcholine receptors. Biochem 17: 2308-2313.
Lukas RJ, Bennett EL (1978) (alpha) Bungarotoxin binding properties of a central nervous system nicotinic acetylcholine receptor. Biochim Biophys Acta 544: 294-308.
Lukas RJ, Morimoto H, Bennett EL (1979) Effects of thio group modification and Ca2+ on agonist specific state transitions of a central nicotinic acetylcholine receptor. Biochem 18: 2384-2395.
Lukas RJ, Bennett EL (1979) Agonist induced affinity alterations of a central nervous system (alpha) ?bungarotoxin receptor. J Neurochem 33: 1151-1157.
Lukas RJ, Bennett EL (1979) Effects of local anesthetics on cholinergic agonist binding affinity of central nervous system (alpha) bungarotoxin receptors. FEBS Lett 108: 356-358.
Lukas RJ, Bennett EL (1980) Interaction of nicotinic receptor affinity reagents with central nervous system (alpha) bungarotoxin binding entities. Molec Pharm 17: 149-155.
Lukas RJ, Bennett EL (1980) Chemical modification and reactivity of sulfhydryls and disulfides of rat brain nicotinic like acetylcholine receptors. J Biol Chem 255: 5573-5577.
Walker JW, Lukas RJ, McNamee MG (1981) Effects of thio group modifications on the ion permeability control and ligand binding properties of Torpedo californica acetylcholine receptor. Biochem 20: 2191-2199.
Lukas RJ, Morimoto H, Hanley MR, Bennett EL (1981) Radiolabeled (alpha) bungarotoxin derivatives: Kinetic interaction with nicotinic acetylcholine receptors. Biochem 20: 7373-7378.
Lukas RJ (1984) Properties of curaremimetic neurotoxin binding sites in the rat central nervous system. Biochem 23: 1152-1160.
Lukas RJ (1984) Detection of low affinity (alpha) bungarotoxin binding sites in the rat central nervous system. Biochem 23: 1160-1164.
Lukas RJ (1984) Monoclonal rat anti-Torpedo electroplax nicotinic acetylcholine receptor antibodies: Immunochemical characterization. J Immuno Meth 74: 129-138.
Lukas RJ (1986) Characterization of curaremimetic neurotoxin binding sites on membrane fractions derived from the human medulloblastoma clonal line, TE671. J Neurochem 46: 1936-1941.
Siegel HN, Lukas RJ (1986) Allosteric modification of (alpha) bungarotoxin binding by the "calcium channel antagonist" verapamil. Molec Brain Res 1: 37-42.
Lukas RJ (1986) Interactions of polyclonal anti-Electrophorus nicotinic receptor antisera with Torpedo nicotinic receptor. Intl J Biochem 18: 609-616.
Lukas RJ (1986) Immunochemical and pharmacological distinctions between curaremimetic neurotoxin binding sites of central, autonomic, and peripheral origin. Proc Natl Acad Sci, USA 83: 5741-5745.
Lukas RJ (1986) Interactions of anti-nicotinic acetylcholine receptor antibodies with rat brain and muscle antigenic determinants. Cellular Molec Neurobiol 6: 281-289.
Lukas RJ (1986) Characterization of curaremimetic neurotoxin binding sites on cellular membrane fragments derived from the rat pheochromocytoma PC12. J Neurochem 47: 1768-1773.
Lukas RJ (1986) Interactions of anti-nicotinic acetylcholine receptor antibodies with (alpha) bungarotoxin binding sites across species and tissues. Molec Brain Res 1: 119-125.
Lukas RJ (1988) Evidence for functional and structural diversity of nicotinic acetylcholine receptors. NATO ASI Series (Clementi F, Gotti C, Sher E, eds) Vol H25: 61-75.
Siegel HN, Lukas RJ (1988) Nicotinic agonists regulate (alpha) bungarotoxin binding sites of TE671 human medulloblastoma cells. J Neurochem 50: 1272-1278.
Siegel HN, Lukas RJ (1988) Morphological and biochemical differentiation of the human medulloblastoma clonal line TE671. Dev Brain Res 44: 269-280.
Lukas RJ, Cullen MJ (1988) An isotopic rubidium ion efflux assay for the functional characterization of nicotinic acetylcholine receptors on clonal cell lines. Analyt Biochem 175: 212-218.
Lukas RJ (1989) Nicotinic acetylcholine receptor diversity: Agonist binding and functional potency. Prog Brain Res (Nordberg A, Fuxe K, Holmstedt B, eds) Vol 79: 117-127.
Oswald RE, Papke RL, Lukas RJ (1989) Characterization of nicotinic acetylcholine receptor channels on the TE671 human medulloblastoma clonal line. Neurosci Lett 96: 207-212.
Lukas RJ (1989) Pharmacological distinctions between functional nicotinic acetylcholine receptors on the PC12 rat pheochromocytoma and the TE671 human medulloblastoma. J Pharm Exper Thera 251: 175-182.
Lukas RJ (1990) Neurotransmitter receptor diversity: The nicotinic acetylcholine receptor family. BNI Quarterly 6: 15-23.
Lukas RJ (1990) Heterogeneity of high affinity nicotinic 3H acetylcholine binding sites. J Pharm Exper Thera 253: 51-57
Lukas RJ (1991) Effects of chronic nicotinic ligand exposure on functional activity of nicotinic acetylcholine receptors expressed by cells of the PC12 rat pheochromocytoma or the TE671/RD human clonal lines. J Neurochem 56: 1134-1145.
Bencherif M, Lukas RJ (1991) Differential regulation of nicotinic acetylcholine receptor expression by human TE671/RD cells following second messenger modulation and sodium butyrate treatments. Molec Cellular Neurosci 2: 52-65.
Bencherif M, Lukas RJ (1991) Ligand binding and functional characterization of muscarinic acetylcholine receptors on the TE671/RD cell line. J Pharmacol Exper Thera 257: 946-953.
Bencherif M, Lukas RJ (1991) Differential sensitivity of phosphoinositide metabolism to sodium fluoride and carbachol treatments in PC12 cells. Molec Cellular Neurosci 2: 377-383.
Lukas RJ, Bencherif M (1992) Heterogeneity and regulation of nicotinic acetylcholine receptors. Intl Rev Neurobiol 34: 25-131.
Bencherif M, Lukas RJ (1992) Vanadate amplifies receptor mediated accumulation of inositol trisphosphates and inhibits inositol tris and tetrakis phosphatase activities. Neurosci Lett 134: 157-160.
Lukas RJ, Norman SA, Lucero L (1993) Characterization of nicotinic acetylcholine receptors expressed by cells of the SH SY5Y human neuroblastoma clonal line. Molec Cellular Neurosci 4: 1-12.
Joy AM, Siegel HN, Lukas RJ (1993) Photoaffinity labeling of muscle type nicotinic acetylcholine receptors and neuronal/nicotinic (alpha) bungarotoxin binding sites with a derivative of (alpha) bungarotoxin. Molec Brain Res 17: 95-100.
Lukas RJ (1993) Expression of ganglia type nicotinic acetylcholine receptors and nicotinic ligand binding sites by cells of the IMR 32 human neuroblastoma clonal line. J Pharm Exper Thera 265: 294-302.
Puchacz E, Stachowiak EK, Florkiewicz RZ, Lukas RJ, Stachowiak MK (1993) Basic fibroblast growth factor (bFGF) regulates tyrosine hydroxylase and proenkephalin mRNA levels in adrenal chromaffin cells. Brain Res 610: 39-52.
Bencherif M, Lukas RJ (1993) Cytochalasin modulation of nicotinic cholinergic receptor expression and muscarinic receptor function in human TE671/RD cells: A possible functional role of the cytoskeleton. J Neurochem 61: 852-864.
Puchacz E, Buisson B, Bertrand D, Lukas RJ (1994) Functional expression of nicotinic acetylcholine receptors containing rat (alpha)7 subunits in human neuroblastoma cells. FEBS Lett 354: 155-159.
Lukas RJ (1995) Diversity and patterns of regulation of nicotinic acetylcholine receptors. Ann N Y Acad Sci 757: 153-168.
Bencherif M, Fowler K, Lukas RJ, Lippiello PM (1995) Mechanisms of upregulation of neuronal nicotinic acetylcholine receptors in clonal cell lines and primary cultures of fetal rat brain. J Pharm Exper Thera 275: 987-994.
Bencherif M, Eisenhour CM, Prince RJ, Lippiello PM, Lukas RJ (1995) The "calcium antagonist" TMB 8 [3,4,5-trimethoxy benzoic acid 8-(diethylamino)octyl ester] is a potent, non-competitive, functional antagonist at diverse nicotinic acetylcholine receptor subtypes. J Pharm Exper Thera 275: 1418-1426.
Quik M, Choremis J, Komourian J, Lukas RJ, Puchacz E (1996) Similarity between rat brain nicotinic (alpha)-bungarotoxin receptors and stably expressed (alpha)-bungarotoxin binding sites. J Neurochem 67: 145-154.
Lukas RJ, Eisenhour CM (1996) Interactions between tachykinins and diverse, human nicotinic acetylcholine receptor subtypes. Neurochem Res 21: 1245-1257.
Ke L, Lukas RJ (1996) Effects of steroid exposure on ligand binding and functional activities of diverse nicotinic acetylcholine receptor subtypes. J Neurochem 67: 1100-1112.
Lukas RJ, Ke L, Bencherif M, Eisenhour CM (1996) Regulation by nicotine of its own receptors. Drug Devel Res 38: 136-148.
Lukas RJ (1998) Neuronal nicotinic acetylcholine receptors. In The Nicotinic Acetylcholine Receptor: Current Views and Future
Trends (Barrantes FJ, ed), Springer Verlag, Berlin/Heidelberg and Landes Publishing Co, Georgetown, Texas, pp 145-173.
Sparks DL, Lukas RJ, Drumm DA (1998) Dementing Lewy body disease: A review of the literature. BNI Quarterly 14: 4-12.
Ke L, Eisenhour CE, Bencherif M, Lukas RJ (1998) Effects of chronic nicotine treatment on expression and function of diverse nicotinic receptor subtypes. I. Dose- and time-dependent effects of nicotine treatment. J Pharmacol Exper Thera 286: 825-840.
Sparks DL, Beach T, Lukas RJ (1998) Immunohistochemical localization of nicotinic receptor (beta)2 and (alpha)4 subunits in normal human brain and individuals with Lewy body and Alzheimer's disease: Preliminary observations. Neurosci Lett 256: 151-154.
Lukas RJ (1999) Cell lines as models for studies of nicotinic acetylcholine receptors. In Neuronal Nicotinic Receptors: Pharmacology and Therapeutic Opportunities (Arneric SP, Brioni JD, eds), Wiley-Liss, Inc, New York, pp 81-97.
Lukas RJ, Changeux J-P, Le Novère N, Albuquerque EX, Balfour DJK, Berg DK, Bertrand D, Chiappinelli VA, Clarke PBS, Collins AC, Dani JA, Grady SR, Kellar KJ, Lindstrom JM, Marks MJ, Quik M, Taylor PW, Wonnacott S (1999) International Union of Pharmacology. XX. Current status of the nomenclature for nicotinic acetylcholine receptors and their subunits. Pharmacol Rev 51: 397-401.
Fryer JD, Lukas RJ (1999) Non-competitive functional inhibition at diverse, human nicotinic acetylcholine receptor subtypes by bupropion, phencyclidine, and ibogaine. J Pharmacol Exper Thera 288: 88-92.
Fryer JD, Lukas RJ (1999) Antidepressants non-competitively inhibit nicotinic acetylcholine receptor function. J Neurochem 72: 1117-1124.
Peng J-H, Lucero L, Fryer J, Herl J, Leonard SS, Lukas RJ (1999) Inducible, heterologous expression of human (alpha)7-neuronal nicotinic acetylcholine receptors in a native nicotinic receptor-null human clonal line. Brain Res 825: 172-179.
Reitstetter R, Lukas RJ, Gruener R. (1999) Dependence of nicotinic acetylcholine receptor recovery from desensitization on the duration of agonist exposure. J Phamacol Exper Thera 289: 656-660.
Sparks DL, Kuo Y-M, Roher A, Martin T, Lukas RJ (2000) Alterations of Alzheimer's disease in the cholesterol-fed rabbit, including vascular inflammation. Preliminary observations. Ann N Y Acad Sci 903: 335-344.
Lukas RJ, Albuquerque EX, Balfour DJK, Clarke PBS, Lindstrom JM, Le Novère N, Quik M, Wonnacott S (2000) Nicotinic acetylcholine receptors. In The IUPHAR Compendium of Receptor Characterization and Classification, IUPHAR Media, London.
Houlihan LM, Slater EY, Beadle DJ, Lukas RJ, Bermudez I (2000) The effects of diltiazem on human nicotinic acetylcholine and GABAA receptors. Neuropharm 39: 2533-2542.
Ferchmin PA, Lukas RJ, Hann RM, Fryer JD, Eaton JB, Pagan OR, Rodriguez AD, Nicolau Y, Rosado Y, Cortes S, Eterovic VA (2001) Tobacco cembranoids block behavioral sensitization to nicotine and inhibit neuronal acetylcholine receptor function. J Neurosci Res 64: 18-25.
Lukas RJ, Lucero L, Buisson B, Galzi J-L, Puchacz E, Fryer JD, Changeux J-P, Bertrand D (2001) Neurotoxicity of channel mutations in heterologously expressed (alpha)7-nicotinic acetylcholine receptors. Eur J Neurosci 13: 1849-1860.
Houlihan LM, Slater EY, Guerra DL, Peng J-H, Kuo, Y-P, Lukas RJ, Cassels BK, Bermudez I (2001) Activity of cytisine and its brominated isosteres on recombinant human (alpha)7, (alpha)4(beta)2 and (alpha)4(beta)4 nicotinic acetylcholine receptors. J Neurochem 78: 1029-1043.
Gentry CL, Lukas RJ (2001) Local anesthetics noncompetitively inhibit function of four distinct nicotinic acetylcholine receptor subtypes. J Pharm Exper Thera 299: 1038-1048.
Lukas RJ, Fryer JD, Eaton JB, Gentry CL (2002) Some methods for studies of nicotinic acetylcholine receptor pharmacology. In Nicotinic Receptors and the Nervous System (Levin ED, ed), CRC Press, Boca Raton, pp 3-27.
Lukas RJ, Tubbs KA, Krivoshein AV, Bieber AL, Nelson RW (2002) Mass spectrometry of nicotinic acetylcholine receptors and associated proteins as models for complex transmembrane proteins. Analyt Biochem 301: 175-188.
Kuo Y-P, Lucero L, Michaels J, DeLuca D, Lukas RJ (2002) Differential expression of nicotinic acetylcholine receptor subunits in fetal and neonatal mouse thymus. J Neuroimmunol 130: 140-154.
Middlebrook AJ, Martina C, Chang Y, Lukas RJ, DeLuca D (2002) Effects of nicotine exposure on developing thymocytes in fetal thymus organ culture. J Immunol 169: 2915-2924.
Gentry CL. Lukas RJ (2002) Regulation of nicotinic acetylcholine receptor numbers and function by chronic nicotine exposure. Current Drug Targets - CNS & Neurological Disorders 1: 359-385.
Shytle RD, Silver AA, Lukas RJ, Newman MB, Sheehan DV, Sanberg PR (2002) Nicotinic acetylcholine receptors as targets for antidepressants. Molec Psychiat 7: 525-535.
Sabbagh MN, Lukas RJ, Sparks DL, Reid RT (2002) The nicotinic acetylcholine receptor, smoking, and Alzheimer's disease. J Alzheimer's Disease 4: 317-325.
Newman MB, Kuo Y-P, Lukas RJ, Shytle RD, Sanberg PR, McGrogan MP Zigova T (2002) Nicotinic acetylcholine receptors on NT2 precursor cells and hNT (NT2-N) neurons. Dev Brain Res 139: 73-86.
Slater Y, Houlihan LM, Cassells BK, Lukas RJ, Bermudez I (2002) Effects of the plant alkaloid tetrandine on human nicotinic acetylcholine receptors. Eur J Pharm 450: 213-221.
Gentry CL, Wilkins LH jr, Lukas RJ (2003) Effects of prolonged nicotinic ligand exposure on function of heterologously expressed, human (alpha)4(beta)2- and (alpha)4(beta)4-nicotinic acetylcholine receptors. J Pharm Exper Thera 304, 206-216.
Dunckley T, Wu J, Zhao L, Lukas RJ (2003) Mutational analysis of roles for extracellular cysteine residues on assembly and function of human (alpha)7-nicotinic acetylcholine receptors. Biochem 42, 870-876.
Dunckley T, Lukas RJ (2003) Nicotine modulates the expression of a diverse set of genes in the neuronal SH-SY5Y cell line. J Biol Chem 278: 16533-15640.
Schroeder KM, Wu J, Zhao L, Lukas RJ (2003) Regulation by cycloheximide and lowered temperature of cell-surface (alpha)7-nicotinic acetylcholine receptor expression on transfected SH-EP1 cells. J Neurochem 85: 581-591.
Slater YE, Houlihan LM, Maskell PD, Exley R, Bermudez I, Lukas RJ, Valdivia AC, Cassels, BK (2003) Halogenated cytisine derivatives as agonists at human neuronal nicotinic acetylcholine receptor subtypes. Neuropharm 44: 503-515.
Zhao L, Kuo Y-P, George AA, Peng J-H, Purandare MS, Schroeder KM, Lukas RJ, Wu J (2003) Functional properties of homomeric, human (alpha)7-nicotinic acetylcholine receptors heterologously expressed in the SH-EP1 human epithelial cell line. J Pharm Exper Thera 305: 1132-1141.
Eaton JB, Peng J-H, Schroeder KM, George AA, Fryer JD, Krishnan C, Buhlman L, Kuo Y-P, Steinlein O, Lukas RJ (2003) Characterization of human (alpha)4(beta)2-nicotinic acetylcholine receptors stably and heterologously expressed in native nicotinic receptor-null SH-EP1 human epithelial cells. Mol Pharm 64: 1283-1294.
Wu J, George AA, Schroeder KM, Xu, L, Marxer-Miller S, Lucero L, Lukas RJ (2004) Electrophysiological, pharmacological and molecular evidence for (alpha)7-nicotinic acetylcholine receptors in rat midbrain dopamine neurons. J Pharm Exper Thera 311: 80-91.
Damaj MI, Carroll FI, Eaton JB, Navarro HA, Blough BE, Mirza S, Lukas RJ, Martin BR (2004) Enantioselective effects of hydroxymetabolites of bupropion on behavior and on function of monoamine transporters and nicotinic receptors. Mol Pharm 66: 675-682.
Wu J, Kuo Y-P, George AA, Xu L, Hu J, Lukas RJ (2004) (alpha)-amyloid directly inhibits human (alpha)4(beta)2-nicotinic acetylcholine receptors heterologously expressed in human SH-EP1 cells. J Biol Chem 279: 37842-37851.
Lukas RJ (2005) Translational impact of basic research studies of nicotinic acetylcholine receptors. Barrow Quarterly 21: 4-12.
Grinevich VP, Letchworth SR, Lindenberger KA, Menager J, Mary V, Sadieva KA, Buhlman LM, Bohme GA, Pradier L, Benavides J, Lukas RJ, Bencherif M (2005) Heterologous expression of human (alpha)6(beta)4(beta)3(alpha)5 nicotinic acetylcholine receptors: Binding properties consistent with their natural expression require quaternary subunit assembly including the (alpha)5 subunit. J Pharm Exper Thera 312: 619-626.
Peng J-H, Fryer JD, Hurst RS, Schroeder KM, George AA, Morrissy S, Groppi V, Leonard SS, Lukas RJ (2005) High-affinity epibatidine binding of functional, human (alpha)7-nicotinic acetylcholine receptors stably and heterologously expressed de novo in human SH-EP1 cells. J Pharm Exper Thera 313: 24-35.
Song C, Murray TA, Kimura R, Wakui M, Ellsworth K, Schroeder KM, Marxer-Miller S, Lukas RJ, Wu J (2005) Role of (alpha)7-nicotinic acetylcholine receptors in titanic stimulation (gamma)-induced oscillations in rat hippocampal slices. Neuropharm 48: 869-880.
Exley R, Iturriaga-Vásquez P, Lukas RJ, Sher E, Cassels BK, Bermudez I (2005) Evaluation of benzyltetrahydroisoquinolines as ligands for brain nicotinic acetylcholine receptors. Br J Pharm 146: 15-24.
Kuo Y-P, Xu L, Eaton JB, Zhao L, Wu J, Lukas RJ (2005) Roles for nicotinic acetylcholine receptor subunit large cytoplasmic loop sequences in receptor expression and function. J Pharm Exper Thera 314: 455-466.
Pakkanen JS, Nousiainen H, Yli-Kauhaluoma J, Kylänlaht I, Moykkynen T, Korpi ER, Peng J-H, Lukas RJ, Ahtee L, Tuominen RK (2005) Methadone increases intracellular calcium in SH-SY5Y and SH-EP1-h(alpah)7 cells by activating neuronal nicotinic receptors. J Neurochem 94: 1329-1341.
Lukas RJ, Wu J, George AA, Kuo Y-P, Xu L, Hu J (2006) b-Amyloid directly inhibits human (alpha)4(beta)2-nicotinic acetylcholine receptors. Revista Brasileira Neurologia, II International Congress on Neuroregeneration (Proceedings), pp 17-20.
Lukas RJ (2006) Potential roles for nicotinic acetylcholine receptors in Parkinson's and Alzheimer's diseases. Revista Brasileira Neurologia, II International Congress on Neuroregeneration (Proceedings), pp 21-29.
Lukas RJ, Bencherif M (2006) Recent developments in nicotinic acetylcholine receptor biology. In Biological and Biophysical Aspects of Ligand-Gated Ion Channel Receptor Superfamilies (Arias H, ed), Research Signpost, Trivandrum, India, pp 27-59.
Lukas RJ (2006) Pharmacological effects of nicotine and nicotinic receptor subtype pharmacological profiles. In Medication Treatments for Nicotine Dependence (George TP, ed), CRC Press, Boca Raton, pp 3-23.
Matta SG, Balfour DJ, Benowitz NL, Boyd RT, Buccafusco JJ, Caggiula AR, Craig CR, Collins AC, Corrigall WA, Damaj MI, Donny EC, Gardiner PS, Grady SR, Heberlein U, Leonard SS, Levin ED, Lukas RJ, Markou A, Marks MJ, McCallum SE, Parameswaran N, Perkins KA, Picciotto MR, Quik M, Rose JE, Rothenfluh A, Schafer WR, Stolerman IP, Tyndale RF, Wehner JM, Zirger JM (2006) Guidelines on nicotine dose selection for in vivo research. Psychopharmacology (Berl). 2006 Aug 9; [Epub ahead of print].
Hu J, Lindenberger K, Hu G, Wang H, Lukas RJ, Wu J (2006) Iptakalim as a human nicotinic acetylcholine receptor antagonist. J Pharm Exper Thera 316: 914-925.
Wu J, Liu Q, Yu K, Hu J, Kuo Y-P, Segerberg M, St. John P, Lukas RJ (2006) Roles of nicotinic acetylcholine receptor (beta) subunits in functional properties of human (alpha)4-containing nicotinic receptors. J Physiol, 2006 Jul 6; [Epub ahead of print].
Dunckley T Lukas RJ (2006) Nicotinic modulation of gene expression in SH-SY5Y neuroblastoma cells. Br Res, in press.
